Course Information
Course Title Code Semester T + P ECTS
Biochemistry Laboratory KIM454 8 0 + 3 3

Prerequisites non

Language Turkish
Level Bachelor's Degree
Type Compulsory
Coordinator Assist.Prof. BURAK ÇOBAN
Instructors Assist.Prof. BURAK ÇOBAN
Goals aim is to give basic biochemistry lab skills to the students
Contents definition of the biomolecules and introducing some biochemical methods.
Work Placement(s) Absent

Number Learning Outcomes
1 students will gain skills on some biochemical lab techniques and also applications of known techniques into biochemistry

Mode of Delivery Face-to-Face
Planned Learning Activities & Teaching Methods lab experiments
Assessment Methods two written exams: mid term and final



Course Content
Week Topics Study Materials
1 lab safety lab notes should be read and researched on the details
2 characteristical reactions of amino acids lab notes should be read and researched on the detail
3 characteristical reactions of amino acids lab notes should be read and researched on the detail
4 enzyme kinetics lab notes should be read and researched on the detail
5 enzyme kinetics of polyphenol oxidases (PPO) lab notes should be read and researched on the detail
6 isolation of cafein from dry tea leaves lab notes should be read and researched on the detail
7 determination of vitamin C in fruit juices lab notes should be read and researched on the detail
8 effects of substrate and enzyme concentration on the enzymatic reactions lab notes should be read and researched on the detail
9 isolation of catalase lab notes should be read and researched on the detail
10 chromatography techniques I (paper chromatography) lab notes should be read and researched on the detail
11 chromatography techniques II (thin layer chromatography - TLC) lab notes should be read and researched on the detail
12 chromatography techniques III (flash column chromatography) lab notes should be read and researched on the detail
13 electrophoresis lab notes should be read and researched on the detail
14 additional lab experiment lab notes should be read and researched on the detail



Sources
Textbook lab notes areon the net
Additional Resources Saadettin Güner, Temel Biyokimya Teknikleri ve Uygulamaları, Esen Ofset M., Trabzon, 2001. Sağıroğlu, A., Yeşiloğlu, Y., Yağar, H., ve İşbilir, Ş., Temel Biyokimya Deneyleri, Trakya Üniversitesi Fen-Edebiyat Fakültesi, Edirne, 2000. Koolman, J., Roehm, K. H., Color Atlas of Biochemistry, second Edition, Thieme, Stuttgart, 2005. Yazgan, Ş. ve K. Büyükgüzel, Biyokimya Laboratuvarı, 1999.



Assessment System Quantity Percentage
In-Term Studies
Mid-terms 1 100
Quizzes 12 20
Laboratory 12 40
In-Term Total 25 160
Contribution of In-Term Studies to Overall 40
Contribution of Final Exam to Overall 60
Total 100





Course's Contribution to PLO
No Key Learning Outcomes Level
1 2 3 4 5
1 Has textbooks containing current information, application tools and equipment , and advanced theoretical and practical knowledge supported by other resources in a scientific approach.
2 Adapts and transfers the acquired knowledge to secondary education.
3 Uses advanced institutional and practical knowledge acquired in the field. x
4 Updates the information on daily conditions.
5 Comments on and evaluate the data by using advanced knowledge and skills acquired in the field, identifies and analyzes the current problems of technological developments, and comes up with solutions based on research and evidence. x
6 Has the ability to conceptualize the events and facts related with the field; analyze them with scientific methods and techniques. x
7 Designs and performs experiments to analyze the problems, collects data, performs analyzes and comment on the results. x
8 Carries out an advanced study related to the field independently. x
9 Takes on responsibility individually and as a team member in order to solve unpredictable and complex problems encountered in applications related to the field. x
10 Plans and manages the activities in a project under his responsibility for development.
11 Plays a role in the process of decision making when faced with problems about different discipline fields.
12 Uses time effectively in the process of inference with the ability of thinking analytically.
13 Evaluates the advanced knowledge and skills acquired in the field with a critical perspective.
14 Determines the learning requirements and leads the learning process.
15 Develops a positive attitude towards lifelong learning.
16 Is aware of the necessity of lifelong learning and develops his Professional knowledge and skills continuously.
17 Informs people and organizations about the topics related to their fields; expresses his ideas and suggestions for solutions to problems in both oral and written form. x
18 Shares his ideas and suggestions for solutions to the problems with experts and non-experts by supporting them with quantitative and qualitative data.
19 Organizes projects and activities for social environment he lives in with an awareness of social responsibility.
20 Follows advances in the field and communicate with colleagues by using a foreign language at least at B1 level of European Language Portfolio.
21 Uses information and communication technology along with software the Human Sciences the field requires at an advanced level.
22 Uses his knowledge of human health and environmental awareness acquired in their fields for society’s ends.
23 Behaves in a way adhering to the social, scientific, cultural and ethical values in the process of data collection, commenting, application, publicizing the results related with the field
24 Has a sufficient level of awareness about the universality of social rights, social justice, quality management, acting in a suitable way in processes and attendance (Instead of quality culture) the protection of cultural values, protection of the environment and health and security in the professional field.



ECTS ALLOCATED BASED ON STUDENT WORKLOAD BY THE COURSE DESCRIPTION
Activities Quantity Duration (Hour) Total Work Load (h)
Hours for off-the-classroom study (Pre-study, practice) 14 3 42
Laboratory 12 3 36
Mid-terms 1 2 2
Final examination 1 2 2
Total Work Load (h) 82
Total Work Load / 30 (h) 2.73
ECTS Credit of the Course 3