Course Information
Course Title Code Semester T + P ECTS
Chromatographic Separation Techniques KIM414 8 2 + 0 3

Prerequisites No

Language Turkish
Level Bachelor's Degree
Type Area Elective
Coordinator Assist.Prof. HASAN ÇABUK
Instructors Assist.Prof. HASAN ÇABUK
Goals Provide information on methods and applications of chromatographic separation
Contents Introduction and basic concepts of chromatography, Introduction to the chromatography techniques,Chromatographic separation theory and mathematical expressions,Thin Layer Chromatography(TLC), Column Chromatography, Gas chromatography, injection systems, Columns and detectors used in gas chromatography, the fields of application, Gas chromatography / mass spectrometry, High performance liquid chromatography (HPLC) injection systems, pump systems, Columns and detectors used in High-performance liquid chromatography (HPLC) , the fields of application, Super Critical Fluid Chromatography, fields of application, Electrophoresis
Work Placement(s) Absent

Number Learning Outcomes
1 Knowledge of chromatography, the chromatographic method in which to learn how to implement

Mode of Delivery Face-to-Face
Planned Learning Activities & Teaching Methods Homework
Assessment Methods Midterms, Final Exam



Course Content
Week Topics Study Materials
1 Introduction and basic concepts of chromatography Students study related subject from sources and then join to lesson.
2 Introduction to the chromatography techniques Students study related subject from sources and then join to lesson.
3 Introduction to the chromatography techniques Students study related subject from sources and then join to lesson.
4 Chromatographic separation theory and mathematical expressions Students study related subject from sources and then join to lesson.
5 Thin Layer Chromatography (TLC) Students study related subject from sources and then join to lesson.
6 Column Chromatography Students study related subject from sources and then join to lesson.
7 Gas chromatography, injection systems Students study related subject from sources and then join to lesson.
8 Midterms Subjects which include midterm exam, solved examples and problems in the class are restudied and, particularly,other various problems are tried to solve with skills that is gained from solved examples.
9 Columns and detectors used in gas chromatography, the fields of application. Students study related subject from sources and then join to lesson.
10 Gas chromatography / mass spectrometry Students study related subject from sources and then join to lesson.
11 High performance liquid chromatography (HPLC) injection systems, pump systems Students study related subject from sources and then join to lesson.
12 Columns and detectors used in High-performance liquid chromatography (HPLC) , the fields of application Students study related subject from sources and then join to lesson.
13 Super Critical Fluid Chromatography, fields of application Students study related subject from sources and then join to lesson.
14 Electrophoresis Students study related subject from sources and then join to lesson.



Sources
Textbook Skoog*Holler*Nieman, Instrumental analysis book
Additional Resources Other Instrumental analysis books



Assessment System Quantity Percentage
In-Term Studies
Mid-terms 1 100
In-Term Total 1 100
Contribution of In-Term Studies to Overall 40
Contribution of Final Exam to Overall 60
Total 100





Course's Contribution to PLO
No Key Learning Outcomes Level
1 2 3 4 5
1 Has textbooks containing current information, application tools and equipment , and advanced theoretical and practical knowledge supported by other resources in a scientific approach. x
2 Adapts and transfers the acquired knowledge to secondary education. x
3 Uses advanced institutional and practical knowledge acquired in the field. x
4 Updates the information on daily conditions. x
5 Comments on and evaluate the data by using advanced knowledge and skills acquired in the field, identifies and analyzes the current problems of technological developments, and comes up with solutions based on research and evidence. x
6 Has the ability to conceptualize the events and facts related with the field; analyze them with scientific methods and techniques. x
7 Designs and performs experiments to analyze the problems, collects data, performs analyzes and comment on the results. x
8 Carries out an advanced study related to the field independently. x
9 Takes on responsibility individually and as a team member in order to solve unpredictable and complex problems encountered in applications related to the field. x
10 Plans and manages the activities in a project under his responsibility for development. x
11 Plays a role in the process of decision making when faced with problems about different discipline fields. x
12 Uses time effectively in the process of inference with the ability of thinking analytically. x
13 Evaluates the advanced knowledge and skills acquired in the field with a critical perspective. x
14 Determines the learning requirements and leads the learning process. x
15 Develops a positive attitude towards lifelong learning. x
16 Is aware of the necessity of lifelong learning and develops his Professional knowledge and skills continuously. x
17 Informs people and organizations about the topics related to their fields; expresses his ideas and suggestions for solutions to problems in both oral and written form. x
18 Shares his ideas and suggestions for solutions to the problems with experts and non-experts by supporting them with quantitative and qualitative data. x
19 Organizes projects and activities for social environment he lives in with an awareness of social responsibility. x
20 Follows advances in the field and communicate with colleagues by using a foreign language at least at B1 level of European Language Portfolio. x
21 Uses information and communication technology along with software the Human Sciences the field requires at an advanced level. x
22 Uses his knowledge of human health and environmental awareness acquired in their fields for society’s ends. x
23 Behaves in a way adhering to the social, scientific, cultural and ethical values in the process of data collection, commenting, application, publicizing the results related with the field x
24 Has a sufficient level of awareness about the universality of social rights, social justice, quality management, acting in a suitable way in processes and attendance (Instead of quality culture) the protection of cultural values, protection of the environment and health and security in the professional field. x



ECTS ALLOCATED BASED ON STUDENT WORKLOAD BY THE COURSE DESCRIPTION
Activities Quantity Duration (Hour) Total Work Load (h)
Course Duration 14 3 42
Hours for off-the-classroom study (Pre-study, practice) 14 1 14
Assignments 1 5 5
Presentation / Preparing Seminar 1 5 5
Mid-terms 1 12 12
Final examination 1 12 12
Total Work Load (h) 90
Total Work Load / 30 (h) 3
ECTS Credit of the Course 3